Review



biotinylated fab secondary antibodies  (Jackson Immuno)


Bioz Verified Symbol Jackson Immuno is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Jackson Immuno biotinylated fab secondary antibodies
    Biotinylated Fab Secondary Antibodies, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+fab+secondary+antibodies/Biotin-SP+(long+spacer)+AffiniPure+Fab+Fragment+Goat+Anti-Mouse+IgG/bio_rxiv__64898__2026__03__22__713396-224-29-36
    Average 94 stars, based on 39 article reviews
    biotinylated fab secondary antibodies - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: The fast diffusion of NKCC1 along the axon is driven by glutamatergic activity
    Article Snippet: After washes, cells for NKCC1 detetcion were incubated for 1 min with F(ab’)2-Goat anti-Rabbit IgG (H+L) Secondary Antibody QDot emitting at 655 nm (1 nM; Invitrogen) in PBS (1 M; Invitrogen) supplemented with 10% Casein (v/v) (Sigma). .. Cells for KCC2 detection were incubated for 3-5 min at 37°C with biotinylated Fab secondary antibodies (goat antimouse: 1:700; Jackson Immuno research, cat #115-067-003, West Grove, USA) in imaging medium. .. After washes, cells were incubated for 1 min with streptavidin-coated quantum dots (QDs) emitting at 605 nm (1 nM; Invitrogen) in borate buffer (50 mM) supplemented with sucrose (200 mM) or in PBS (1 M; Invitrogen) supplemented with 10% Casein (v/v) (Sigma).

    Article Title: Conformational state-dependent regulation of GABA A receptor diffusion and subsynaptic domains
    Article Snippet: Briefly, cells were incubated for 3–6minat 37°C with primary antibodies against extracellular epitopes of GABA A R γ2 subunit (guinea pig: 1:800/1:1000, rabbit: 1:500, Synaptic Systems) or GABA A R α1 subunit (rabbit: 1:500, Synaptic Systems) and washed. .. Cells were then incubated for 3–5minat 37°C with biotinylated Fab secondary antibodies (goat anti-guinea pig (106-066-003) or goat anti-rabbit (111-067-003),1:500; Jackson Immunoresearch, West Grove, USA) in imaging medium. .. After washes, cells were incubated for 1 min with streptavidin-coated quantum dots (QDs) (1 nM; Invitrogen, Q10123MP) or anti-rabbit F(ab’)2-QDs emitting at 655 nm (1 nM; Invitrogen, 10592815) in PBS (1X; Invitrogen) in the presence of 10% Casein (v/v, Sigma) to prevent non-specific binding.

    Article Title: GABA A receptor dependent synaptic inhibition rapidly tunes KCC2 activity via the Cl − -sensitive WNK1 kinase
    Article Snippet: .. Briefly, cells were incubated for 3–5 min at 37 °C with primary antibodies against Flag (mouse, 1:700, Sigma, cat #F3165), washed, and incubated for 3–5 min at 37 °C with biotinylated Fab secondary antibodies (goat anti-mouse: 1:700; Jackson Immuno research, cat #115-067-003, West Grove, USA) in imaging medium. .. After washes, cells were incubated for 1 min with streptavidin-coated quantum dots (QDs) emitting at 605 nm (1 nM; Invitrogen) in borate buffer (50 mM) supplemented with sucrose (200 mM) or in PBS (1 M; Invitrogen) supplemented with 10% Casein (v/v) (Sigma).

    Article Title: NKCC1 and KCC2 Chloride Transporters Have Different Membrane Dynamics on the Surface of Hippocampal Neurons.
    Article Snippet: After several washes with the imaging medium, the cells for NKCC1 detection were incubated for 1 min with F(ab’)2-Goat anti-Rabbit IgG (H + L) Secondary Antibody QDot emitting at 655 nm (1 nM; Invitrogen, Cat#10592815) in PBS (1 M; Invitrogen) supplemented with 10% Casein (v/v) (SigmaAldrich). .. The cells for KCC2 detection were incubated for 3–5 min at 37 ◦C with biotinylated Fab secondary antibodies (goat anti-mouse: 1:700; Jackson Immuno research, Montlucon, France, Cat#115-067-003, West Grove, PA, USA) in imaging medium. .. After washes, cells were incubated for 1 min with QdotTM 655 Streptavidin Conjugate (1 nM; Thermo Fisher Scientific, Les Ulis, France, Cat#Q10123MP) in borate buffer (50 mM) supplemented with sucrose (200 mM) or in PBS (1 M; Invitrogen) supplemented with 10% Casein (v/v) (Sigma).

    Article Title: NKCC1 as a signaling hub regulating KCC2 stability, chloride homeostasis, and seizure susceptibility
    Article Snippet: .. Briefly, cells were incubated for 6 min at 37 °C with primary antibodies against Flag (mouse, 1:300, Sigma, cat #F3165) and against HA antibody (rabbit, 1:300, Cell signaling Technology, cat #C29F4), washed, and incubated for 6 min at 37°C with biotinylated Fab secondary antibodies (goat anti-mouse: 1:700; Jackson Immunoresearch, cat #115-067-003, West Grove, USA) and CY3-conjugated donkey anti-rabbit secondary antibodies (1:300, 711-165-152, Jackson Immunoresearch) in imaging medium. .. After washes, cells were incubated for 1 min with streptavidin-coated quantum dots (QDs) emitting at 655nm (1nM; Invitrogen ref Q10123MP) in PBS (1M; Invitrogen) supplemented with 10% Casein (v/v) (Sigma) to prevent non-specific binding.

    Article Title: NKCC1 and KCC2 Chloride Transporters Have Different Membrane Dynamics on the Surface of Hippocampal Neurons
    Article Snippet: After several washes with the imaging medium, the cells for NKCC1 detection were incubated for 1 min with F(ab’)2-Goat anti-Rabbit IgG (H + L) Secondary Antibody QDot emitting at 655 nm (1 nM; Invitrogen, Cat#10592815) in PBS (1 M; Invitrogen) supplemented with 10% Casein ( v / v ) (Sigma-Aldrich). .. The cells for KCC2 detection were incubated for 3–5 min at 37 °C with biotinylated Fab secondary antibodies (goat anti-mouse: 1:700; Jackson Immuno research, Montlucon, France, Cat#115-067-003, West Grove, PA, USA) in imaging medium. .. After washes, cells were incubated for 1 min with QdotTM 655 Streptavidin Conjugate (1 nM; Thermo Fisher Scientific, Les Ulis, France, Cat#Q10123MP) in borate buffer (50 mM) supplemented with sucrose (200 mM) or in PBS (1 M; Invitrogen) supplemented with 10% Casein ( v / v ) (Sigma).

    Article Title: Ganglioside GM1-enriched rafts regulate the neuronal chloride co-transporter KCC2
    Article Snippet: .. Neurons were incubated for 6 min at 37 °C with primary antibodies against Flag (mouse, 1:300, Sigma, cat #F3165), washed, and incubated for 6 min at 37 °C with biotinylated Fab secondary antibodies (goat anti-mouse, 1:300; Jackson Immuno research, cat #115-067-003, West Grove, USA) in imaging medium. .. After washes, cells were incubated for 1 min with streptavidin-coated quantum dots (QDs) emitting at 655 nm (1 nM; Invitrogen) in PBS (1 M; Invitrogen) supplemented with 10% Casein (v/v) (Sigma).

    Imaging:

    Article Title: The fast diffusion of NKCC1 along the axon is driven by glutamatergic activity
    Article Snippet: After washes, cells for NKCC1 detetcion were incubated for 1 min with F(ab’)2-Goat anti-Rabbit IgG (H+L) Secondary Antibody QDot emitting at 655 nm (1 nM; Invitrogen) in PBS (1 M; Invitrogen) supplemented with 10% Casein (v/v) (Sigma). .. Cells for KCC2 detection were incubated for 3-5 min at 37°C with biotinylated Fab secondary antibodies (goat antimouse: 1:700; Jackson Immuno research, cat #115-067-003, West Grove, USA) in imaging medium. .. After washes, cells were incubated for 1 min with streptavidin-coated quantum dots (QDs) emitting at 605 nm (1 nM; Invitrogen) in borate buffer (50 mM) supplemented with sucrose (200 mM) or in PBS (1 M; Invitrogen) supplemented with 10% Casein (v/v) (Sigma).

    Article Title: Conformational state-dependent regulation of GABA A receptor diffusion and subsynaptic domains
    Article Snippet: Briefly, cells were incubated for 3–6minat 37°C with primary antibodies against extracellular epitopes of GABA A R γ2 subunit (guinea pig: 1:800/1:1000, rabbit: 1:500, Synaptic Systems) or GABA A R α1 subunit (rabbit: 1:500, Synaptic Systems) and washed. .. Cells were then incubated for 3–5minat 37°C with biotinylated Fab secondary antibodies (goat anti-guinea pig (106-066-003) or goat anti-rabbit (111-067-003),1:500; Jackson Immunoresearch, West Grove, USA) in imaging medium. .. After washes, cells were incubated for 1 min with streptavidin-coated quantum dots (QDs) (1 nM; Invitrogen, Q10123MP) or anti-rabbit F(ab’)2-QDs emitting at 655 nm (1 nM; Invitrogen, 10592815) in PBS (1X; Invitrogen) in the presence of 10% Casein (v/v, Sigma) to prevent non-specific binding.

    Article Title: GABA A receptor dependent synaptic inhibition rapidly tunes KCC2 activity via the Cl − -sensitive WNK1 kinase
    Article Snippet: .. Briefly, cells were incubated for 3–5 min at 37 °C with primary antibodies against Flag (mouse, 1:700, Sigma, cat #F3165), washed, and incubated for 3–5 min at 37 °C with biotinylated Fab secondary antibodies (goat anti-mouse: 1:700; Jackson Immuno research, cat #115-067-003, West Grove, USA) in imaging medium. .. After washes, cells were incubated for 1 min with streptavidin-coated quantum dots (QDs) emitting at 605 nm (1 nM; Invitrogen) in borate buffer (50 mM) supplemented with sucrose (200 mM) or in PBS (1 M; Invitrogen) supplemented with 10% Casein (v/v) (Sigma).

    Article Title: NKCC1 and KCC2 Chloride Transporters Have Different Membrane Dynamics on the Surface of Hippocampal Neurons.
    Article Snippet: After several washes with the imaging medium, the cells for NKCC1 detection were incubated for 1 min with F(ab’)2-Goat anti-Rabbit IgG (H + L) Secondary Antibody QDot emitting at 655 nm (1 nM; Invitrogen, Cat#10592815) in PBS (1 M; Invitrogen) supplemented with 10% Casein (v/v) (SigmaAldrich). .. The cells for KCC2 detection were incubated for 3–5 min at 37 ◦C with biotinylated Fab secondary antibodies (goat anti-mouse: 1:700; Jackson Immuno research, Montlucon, France, Cat#115-067-003, West Grove, PA, USA) in imaging medium. .. After washes, cells were incubated for 1 min with QdotTM 655 Streptavidin Conjugate (1 nM; Thermo Fisher Scientific, Les Ulis, France, Cat#Q10123MP) in borate buffer (50 mM) supplemented with sucrose (200 mM) or in PBS (1 M; Invitrogen) supplemented with 10% Casein (v/v) (Sigma).

    Article Title: NKCC1 as a signaling hub regulating KCC2 stability, chloride homeostasis, and seizure susceptibility
    Article Snippet: .. Briefly, cells were incubated for 6 min at 37 °C with primary antibodies against Flag (mouse, 1:300, Sigma, cat #F3165) and against HA antibody (rabbit, 1:300, Cell signaling Technology, cat #C29F4), washed, and incubated for 6 min at 37°C with biotinylated Fab secondary antibodies (goat anti-mouse: 1:700; Jackson Immunoresearch, cat #115-067-003, West Grove, USA) and CY3-conjugated donkey anti-rabbit secondary antibodies (1:300, 711-165-152, Jackson Immunoresearch) in imaging medium. .. After washes, cells were incubated for 1 min with streptavidin-coated quantum dots (QDs) emitting at 655nm (1nM; Invitrogen ref Q10123MP) in PBS (1M; Invitrogen) supplemented with 10% Casein (v/v) (Sigma) to prevent non-specific binding.

    Article Title: NKCC1 and KCC2 Chloride Transporters Have Different Membrane Dynamics on the Surface of Hippocampal Neurons
    Article Snippet: After several washes with the imaging medium, the cells for NKCC1 detection were incubated for 1 min with F(ab’)2-Goat anti-Rabbit IgG (H + L) Secondary Antibody QDot emitting at 655 nm (1 nM; Invitrogen, Cat#10592815) in PBS (1 M; Invitrogen) supplemented with 10% Casein ( v / v ) (Sigma-Aldrich). .. The cells for KCC2 detection were incubated for 3–5 min at 37 °C with biotinylated Fab secondary antibodies (goat anti-mouse: 1:700; Jackson Immuno research, Montlucon, France, Cat#115-067-003, West Grove, PA, USA) in imaging medium. .. After washes, cells were incubated for 1 min with QdotTM 655 Streptavidin Conjugate (1 nM; Thermo Fisher Scientific, Les Ulis, France, Cat#Q10123MP) in borate buffer (50 mM) supplemented with sucrose (200 mM) or in PBS (1 M; Invitrogen) supplemented with 10% Casein ( v / v ) (Sigma).

    Article Title: Ganglioside GM1-enriched rafts regulate the neuronal chloride co-transporter KCC2
    Article Snippet: .. Neurons were incubated for 6 min at 37 °C with primary antibodies against Flag (mouse, 1:300, Sigma, cat #F3165), washed, and incubated for 6 min at 37 °C with biotinylated Fab secondary antibodies (goat anti-mouse, 1:300; Jackson Immuno research, cat #115-067-003, West Grove, USA) in imaging medium. .. After washes, cells were incubated for 1 min with streptavidin-coated quantum dots (QDs) emitting at 655 nm (1 nM; Invitrogen) in PBS (1 M; Invitrogen) supplemented with 10% Casein (v/v) (Sigma).

    other:

    Article Title: GABA A receptor dependent synaptic inhibition rapidly tunes KCC2 activity via the Cl - -sensitive WNK1 kinase.
    Article Snippet: To obtain N-terminal HA-tagged WNK3, the purified PCR fragments were directionally assembled to a pCAGGS-HA linearized plasmid using In-Fusion HD cloning kit (Clontech) following manufacturer’s guidelines.



    Similar Products

    94
    Jackson Immuno biotinylated fab secondary antibodies
    Biotinylated Fab Secondary Antibodies, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+fab+secondary+antibodies/Biotin-SP+(long+spacer)+AffiniPure+Fab+Fragment+Goat+Anti-Mouse+IgG/bio_rxiv__64898__2026__03__22__713396-224-29-36
    Average 94 stars, based on 1 article reviews
    biotinylated fab secondary antibodies - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    95
    Boster Bio biotinylated goat fab fragment anti rabbit igg
    Biotinylated Goat Fab Fragment Anti Rabbit Igg, supplied by Boster Bio, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+fab+secondary+antibodies/Rabbit+Anti-Goat+IgG+(H%2BL)+Secondary+Antibody%2C+Biotin+Conjugated/pm40541638-173-150-157
    Average 95 stars, based on 1 article reviews
    biotinylated goat fab fragment anti rabbit igg - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    96
    Boster Bio biotinylated goat fab fragment anti mouse igg
    Biotinylated Goat Fab Fragment Anti Mouse Igg, supplied by Boster Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+fab+secondary+antibodies/Goat+Anti-Mouse+IgG+(H%2BL)+Secondary+Antibody%2C+Biotin+Conjugated/pm40541638-173-51-58
    Average 96 stars, based on 1 article reviews
    biotinylated goat fab fragment anti mouse igg - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    93
    Jackson Immuno biotinylated donkey anti goat secondary antibody
    Biotinylated Donkey Anti Goat Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+fab+secondary+antibodies/Biotin-SP+(long+spacer)+AffiniPure+Fab+Fragment+Donkey+Anti-Goat+IgG/pm39179163-87-19-24
    Average 93 stars, based on 1 article reviews
    biotinylated donkey anti goat secondary antibody - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    93
    Jackson Immuno biotinylated donkey anti rabbit secondary antibody
    Biotinylated Donkey Anti Rabbit Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+fab+secondary+antibodies/Biotin-SP+(long+spacer)+AffiniPure+Fab+Fragment+Donkey+Anti-Rabbit+IgG/pmc11244552-273-20-29
    Average 93 stars, based on 1 article reviews
    biotinylated donkey anti rabbit secondary antibody - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    Image Search Results